पाठशाला भीतर और बाहर का दिसम्बर अंक केन्द्रित है ​‘प्रारम्भिक बाल्यावस्था और शिक्षा’ पर। इस अंक में प्रारम्भिक बाल्यावस्था और शिक्षा पर केन्द्रित विविध अनुभव-आधारित आलेख, एक सैद्धान्तिक लेख, शिक्षकों की डायरी में दर्ज उनके अनुभव उन्हीं की कलम से, कुछ रोचक तथा आसानी से की जा सकने वाली गतिविधियाँ और ऐसी किताबें, जिनका उपयोग प्रारम्भिक बाल्यावस्था के लिए किया जा सके, शामिल हैं। साथ ही ​‘उम्मीद जगाते शिक्षक’ के अन्तर्गत एक आँगनवाड़ी कार्यकर्त्री, जिन्होंने अपने केन्द्र को बदलाव के नए स्तर दिए, की यात्रा के अनुभव, उनकी कहानी भी है। 

Urban blue spaces, such as lakes and rivers, are increasingly recognised for their ecological and social roles, yet their contributions to sustainable food systems remain understudied. Here, the researchers examine the extent and benefits of foraging in urban blue spaces across four major Indian cities through a survey of 1,200 users. We identify three forager groups, that is, ​‘rare’, ​‘occasional’ and ​‘frequent’ foragers, whose behaviours differ in frequency and practice. Women, the elderly and marginalised communities most frequently collect, share, cook and sell edibles. Access to home or community gardens strongly motivates occasional foragers. Frequent foragers emphasise benefits relating to nutrition and income, as well as culture and social capital, whereas occasional foragers appreciate nature- and culture-related benefits. The findings challenge conventional perspectives on urban food provisioning, highlighting urban blue spaces as vital yet overlooked spaces for food access and resilience. Integrating foraging into urban planning can enhance equitable food systems, fostering transformative change toward sustainable urban landscapes.

Authors: Sukanya Basu, Brenda Maria Zoderer, Harini Nagendra, Peter H Verburg, Tobias Plieninger 

This chapter critically examines the different conceptions and emerging paradigms in the discourse on inclusion in the context of education in India. The researcher interrogates the nature of inclusive education and argues for an epistemology that emerges from the paradoxes, diversity and disparities that characterise schools and classrooms in the Indian context. In doing so, the researcher scrutinises the emerging trends in education research and the ​‘new’ epistemology from the global North which attributes agency to the practitioner, the parent and the child to participate in the education discourse, shifting the equation of power in the construction of knowledge. In this chapter, she examines the connotations of these new, emerging trends for research, practice, and policy on inclusion for India. The chapter presents the tensions in arriving at conceptions of inclusive education and how that has impacted policy and its realisation in practice. The central thesis of the paper is constructed through a close examination of the different forms of marginalisation that characterise Indian classrooms, the situation of the disadvantaged child, the parent and the teacher in the context of education. 

The November 2025 issue of At Right Angles takes a look at the annual celebration of the National Day of Mathematics on December 22. Celebrating mathematics makes perfect sense to some, far less to others- with the articles in this issue, we hope to make persuasive arguments that cause shift towards those who love patterns, who enjoy discovery, who believe in celebrating reason, elegant arguments – in short, Mathematics!

The need to strengthen comprehensive primary health care towards ensuring ​“Health for All” is well established yet operationalising this has remained a challenge globally as well as in India. Based on a qualitative study of a collaborative initiative between the government and a civil society organisation, this article discusses what factors and processes explain successful implementation of primary health care in a remote rural area in central India.

Authors: Arima Mishra, Raman Kataria, Roseline Sagar, Pawan Singh, Pankaj Tiwari, Shivkant Tripathi, Vinay Vishwakarma, Sapna Mishra

The Shakti scheme, launched by the Government of Karnataka in June 2023, represents one of the most ambitious efforts in India to reimagine welfare by providing free public transport for women. Unlike welfare programmes based on targeted cash transfers, this scheme redefines inclusion through the provision of a universal, non-cash public service mobility. This report is an assessment of the evolution of the scheme with specific regard to the BMTC’s experience. In the period studied– January 2023 to March 2025, covering both pre- and post-implementation phases – the scheme generated a significant surge in bus usage across the state. With more than 2.89 crore trips recorded, a striking transformation in mobility patterns was observed. Importantly, women riders quickly outnumbered men on many of the busiest routes– especially in the Central Business District, reflecting a major shift in access to and usage of public transport. The ridership gains were particularly sharp in the first six months following the scheme’s launch and have since stabilised, indicating a sustained and regular usage pattern among women. The report is divided into three sections. In the first, the researchers discuss state-level free travel schemes. They address certain questions about Karnataka’s experience within wider debates about universalism versus targeting in social policy. In the second, they discuss the report, its methods and findings. In the final section, they discuss some potential paths forward.

This issue of our magazine brings together articles that point to a simple truth: meaningful reform rests on inclusion and enablement, which calls for an education system that expands possibilities for all learners, strengthens the capacities of teachers and Anganwadi workers, and finally draws on the voices of all stakeholders of the system in shaping the way forward.

पाठशाला भीतर और बाहर का यह विशेष अंक है। इस अंक के साथ पाठशाला पत्रिका ने 25 अंकों का सफ़र तय किया है। इस विशेष 25वें अंक में शामिल हैं 13 राज्यों से आई 25 शिक्षकों की डायरियाँ जिनमें दर्ज हैं उनके कक्षा अनुभव। साथ ही, 5 आलेख ​‘बन्धुता की शिक्षा’, ​‘शिक्षकों के विकास में सहयोग ही उनका सम्मान है’, ​‘शिक्षा और शिक्षक : ज़मीनी चुनौतियों को समझने की जरूरत’, ​‘विद्यार्थियों के जीवन को आकार देते हैं शिक्षक’, ​‘पाठशाला भीतर और बाहर के 25 अंकों का सफ़र’ शामिल हैं। ये आलेख शिक्षकों के काम को भरोसे के तौर पर देखते हैं, और आने वाले समय में शिक्षा में सकारात्मक बदलाव के प्रति आश्वस्ति जगाते हैं। 

Students learn about the properties of matter in Grade VI and its particulate nature in Grade VIII. But can they connect these concepts with observations and experiences of ​‘air’ from their everyday world? Can we use an empty tumbler and an inflated balloon to offer visual evidence of these properties? Explore these questions through the articles and classroom resources in our theme section ​‘Materials Around Us’.

More than just an academic space, the Azim Premji University campus in Bengaluru is fast becoming a thriving ecosystem of its own. Located in Sarjapura, the campus is more than classrooms and labs — it is a habitat shared with birds, reptiles, insects, small mammals, and a growing canopy of trees and plants. This report is a first step in documenting the biodiversity of our campus. From trees and shrubs to spiders and snakes, students of the BSc in Environmental Science and Sustainability, teachers, and other members have come together to observe and record the life that surrounds us. Thus, this is a collective effort that reflects both scientific curiosity and deep care for our environment. As the campus grows, we aim not only to track carbon sequestration and environmental change — but also to nurture a sense of stewardship and belonging in everyone who calls this campus home.

Religion in India is a constantly evolving and transformative entity that cannot be engaged with in isolation from other dimensions of life. This short introduction moves beyond traditional text- and scripture-based academic approaches to provide an overview of the dynamic role of religion in contemporary Indian society. Examining religion through the perspectives of religious actors, interlocutors, and practices, it highlights how religion intersects with the state, secularism, identity, and civic and economic life. An indispensable resource for general readers, students, and researchers, this book will foster grounds for dialogue towards a flourishing of pluralism in present-day India.

Mental health is a global priority, fundamental to the health and development of all nations. The contribution of mental disorders to the global burden of disease is widely recognised; however, a significant care gap exists, particularly in the context of low-and middle-income countries. In India, for instance, there are 0.3 psychiatrists per 1,00,000 population. To address this severe shortage of mental health professionals and resources globally, the World Health Organisation has suggested the adoption of a community-based mental health care approach, where the locus of services shifts from institutional care to local communities. Over the last five decades in India, diverse approaches to mental health care have emerged because of the interaction of dominant discourses on community-based mental health care with various socio-cultural contexts. In addition to the government-run mental health programme and programmes run by medical colleges, civil society organisations have increasingly contributed to this space. Although studies have assessed individual interventions, there exists a need to map these interventions and synthesise the approaches for service delivery to inform public health practice in India and in low-and middle-income countries at large. This narrative review attempts to map and synthesise insights from community-based mental health interventions in India implemented across diverse contexts. The researchers searched peer-reviewed journal articles and book chapters published in the English language between 2010 and 2023. They present the synthesis of approaches used in 41 community-based mental health interventions, where they unpack key intervention components and processes adopted for primary prevention and promotion; identification and case detection; treatment and care, and rehabilitation in the community. This review presents key recommendations for practitioners about the role of community, the diversity and commonalities in various approaches across contexts, the roles of various actors in service delivery, and the shared values guiding the conceptualisation and implementation of community-based mental health interventions in India.

This  case study, documents the trials, tribulations and victories of a citizen’s movement to protect the environment — in this case the Chevella banyans. These banyans  that are of ecological, historical and cultural importance were under threat of being axed for a road widening project. This case study can be used as a teaching resource in undergraduate and postgraduate programmes, and by civil society organisations and citizens as a guiding document to understand how they can contribute to protecting the environment, the strategies they can apply and the challenges they might face.

Authors:

Rini Singhi, Seema Mundoli, Harini Nagendra

The increasing global demand for food, coupled with the impacts of climate change and environmental degradation, has shifted attention towards blue food systems. While these systems offer a potential solution to food security challenges, their sustainability is threatened by various factors, including overfishing, habitat destruction, and climate change. Recognising these challenges, the Food and Agriculture Organisation of the United Nations (FAO, UN) advocates for ensuring the sustainable contribution of aquatic food systems to global food security and nutrition, aligning with the United Nation’s Sustainable Development Goals (UN-SDGs). It was hypothesised that adoption of UN-SDGs would increase the interest of researchers in sustainability of fisheries and aquaculture. Therefore, a bibliometric analysis of the relevant literature published between 2000 and 2023 was conducted to test this hypothesis. The analysis revealed a significant rise in publications addressing climate change and sustainability after the adoption of the UN-SDGs. The countries exhibiting high vulnerability to climate change contributed disproportionately fewer publications to the database. The United States emerged as the leading contributor in terms of publication volume, while Canada’s University of British Columbia was identified as the institution with the highest number of contributing authors. The findings underscore pronounced geographical disparities in research output, with a predominance of countries from the Global North. The underlying factors contributing to these disparities are discussed. Furthermore, the study discusses the policy implications of these findings and emphasises the imperative to prioritise equitable research initiatives. Such efforts are essential to effectively support the realisation of the SDGs within the domain of blue food systems and sustainability.

This report constructs a Rural Multidimensional Deprivation Index (RDI) for measuring deprivation in Jharkhand. An important feature of the index is that it is constructed using indicators on provisioning of basic infrastructural amenities at the village level. This is different from the usual poverty indices that are composed of financial indicators such as income or consumption; or indicators measuring development outcomes such as educational or nutritional levels. Observing the provisioning of basic infrastructural amenities, this report makes focused policy suggestions that can be easily implemented by functionaries working on the ground. The data for the index is sourced from the Mission Antyodaya Survey, which was carried out in 2019 at the village level across the country. The survey collects data related to provisioning and outcome of basic amenities in villages, along with demographic and socioeconomic variables. Because this is a periodic survey, it enables us to track the developmental progress of each village, district, state and the country over time. In total, there are about 182 variables that were captured in the 2019 round. The survey covers 31,175 out of 32,620 villages in Jharkhand, which is 95.6 percent of the total number of villages in the state; and 6,48,358 out of 6,67,933 villages in India, or 97.1 percent of the villages in the country.

Editors

Prasanna S, Sandhya Krishnan, Sanket Gharat, Puja Guha, Amalendu Jyotishi, Neeraj Hatekar

In this chapter, the researchers critically examine the emancipatory role of neoliberalism with a focus on women’s work in India. They argue that the multi-pronged crisis afflicting the labour market is a fallout of the implementation of the neoliberal project in India that had severely impacted women workers, who typically are one of the oppressed sections in society. They argue that neoliberalism leads to the proliferation of social orthodoxies that promote patriarchal gender contracts whereby men are the primary breadwinners while women are mainly responsible for social reproduction in the ​‘domestic’ space.

They highlight that the neoliberal regime in India is associated with declining participation in paid work for women workers, notwithstanding the rapid growth rate of the Gross Domestic Product (GDP) that the economy has been witnessing. This is in the backdrop of India having one of the lowest rates of participation in paid employment of women workers. They claim that women’s work participation in India is affected by the production conditions in Indian agriculture and the burden of unpaid care work. However, increased participation in paid work — the quantitative dimension — does not necessarily lead to women’s empowerment under neoliberalism. This is largely due to the proliferation of the informal sector, which has been the major source of paid work for women workers. The researchers contend that women workers encounter two layers of subordination and control in the labour market that become acute in the informal sector, which is associated with a lack of worker’s rights. They are subordinated, first, by the dominance of capital and, second, by the patriarchal social order. These, in turn, adversely impact the quality and economic value of women’s work. They argue that the degradation of the economic value of women’s work has led to the accentuation of the gender bias that endangers the chances of survival of the girl child in a society characterised by deep-rooted patriarchy.

The contemporary precarity of women’s work — in terms of quantity and quality of work — also reflects the existing social orthodoxies that loom large in the private and public domains in India. Lastly, based on the latest Periodic Labour Force Survey database, they argue that the COVID-19 pandemic has widened the structural inequalities in the economy and added a layer to the existing vulnerabilities of women workers.

Authors: Mampi Bose, Shantanu De Roy

In this book, Prema, a young girl living in Mumbai, learns about heatwaves and their devastating effects when her father falls seriously ill during an intense summer. Through her eyes, readers explore the human impact of rising temperatures and the urgent need for climate awareness.

Authors: Shrunoti Ritesh Tatiya, Santonu Goswami, Seema Mundoli, Harini Nagendra

Illustrations: Shrunoti Ritesh Tatiya

Craft new understanding in primary school mathematics with the July 2025 issue of At Right Angles. And do send in your feedback to AtRightAngles.​editor@​apu.​edu.​in

The Indian economy, despite registering high growth, is characterised by a persistent and vast informal economy. Using it as an illustration, the researchers draw lessons for characterising labour markets in contexts of high informality. They employ a group-based statistical modelling method to identify whether there exist systematic patterns in the high volume of worker transitions across different employment arrangements. Using panel data for eight points between 2017 and 2019, they identify seven dominant labour market trajectories. The trajectory capturing stable formal salaried employment, with highest average earnings, accounts for only 6.7% of the sample. None of the dominant trajectories denote a job ladder from informal to formal work, and the sorting of individuals into informal trajectories is far from voluntary, indicating an existence of formal and informal segmentation. The most populous trajectory, comprising 38.4% of the sample, with second highest average income (although half of that of the formal salaried trajectory), is stable self-employment, followed by the trajectory representing transition within different forms of informal wage work at 27.2%. Most trajectory groups associated with informal wage arrangements have high flux, indicating lack of stability. Furthermore, trajectories associated with informal wage employment have even lower earnings than those with informal self-employment. Far from suggesting a desirability of informal self-employment, this is indicative of a breaking down of the expected voluntary transition from self to wage employment in the transformation process. Additionally, access to trajectories is stratified along various correlates, especially caste. Caste hierarchy operates most starkly at the node of accessing the trajectories, while in terms of penalties or gains in earnings, traditional caste-hierarchy may not always operate uniformly. The findings disrupt the standard expectation in structural transformation models and labour market theories, while highlighting the need to foreground evolving nature of informality in labour market models for developing economies.

Authors: Rosa Abraham, Surbhi Kesar

पाठशाला भीतर और बाहर के जून अंक (24वाँ) में एफ़एलएन की अवधारणा, कक्षा अनुभव, चुनौतियाँ, आदि को केन्द्र में रखकर लिखे गए कुछ लेख हैं। बातचीत के ज़रिए विद्यार्थियों से जुड़ना और उस बातचीत का शिक्षण प्रक्रिया में उपयोग कैसे हो, बच्चों को रचनात्मक लेखन से कैसे जोड़ें, पाठ योजना बनाकर पढ़ाना कैसे उपयोगी होता है, जैसे लेख भी शामिल हैं। एक ऐसे विद्यालय की कहानी को पढ़ना दिलचस्प होगा जिसका नामांकन एकदम कम हो गया था, लेकिन कुछ ख़ास प्रक्रियाएँ अपनाने से उस विद्यालय में न सिर्फ़ नामांकन बढ़ा, बल्कि विद्यार्थियों के सीखने का स्तर भी बेहतर हुआ। 

हमेशा की तरह ईसीसीई पर आलेख है जिसमें आँगनवाड़ी केन्द्र के माहौल और गतिविधियों से जुड़े अनुभव शामिल हैं। नियमित स्तम्भ के अन्तर्गत इस बार ​‘इनसे मिलिए’ में मध्य प्रदेश की शिक्षिका से जानेंगे उनके अनुभव, कि कैसे और कौन‑सी प्रक्रियाओं के चलते सीखना बेहतर हुआ। इसके अलावा, इस अंक में ​‘शिक्षकों की डायरी से’, ​‘किताबों से दोस्ती’, और ​‘आइए, करके देखें’ स्तम्भ भी शामिल हैं।

Play and Early Learning Environment is the first in the series on Education for the Foundational Stage. This thematic volume is a collection of articles on concepts, practices and programmes on play and early learning environment. The papers in the volume provide rich insights on what playful learning looks like in the foundational years in diverse Early Childhood settings and illustrates the ways in which we can develop playful and inclusive learning environments for children.

Editors:

Jigisha Shastri & Kinnari Pandya

This article presents a historical account of emergence and development of the territorial power of Jats in Punjab through analysing three elements. First, their historical appropriation of the village as their agrarian territory, which was often perpetuated under the discourse of ​‘village community’/clans and the monopolisation of the peasant identity. Second, the often-benevolent administration in Punjab during the colonial rule. Third, the development of a discourse of caste-progressiveness of Jats. The Punjab experience in this regard is significant to understand similar processes in other states and to make appropriate strategies for social movements and policy advocacy regarding agrarian/​social reforms.

Résumé

Cet article présente un compte rendu historique de l’émergence et du développement du pouvoir territorial des Jats au Pendjab en analysant trois éléments. Premièrement, leur appropriation historique du village en tant que territoire agraire, qui s’est souvent perpétuée sous le discours de la « communauté villageoise »/​clans et de la monopolisation de l’identité paysanne. Deuxièmement, l’administration souvent bénévole du Pendjab pendant la période coloniale. Troisièmement, le développement d’un discours sur la progressivité de la caste des Jats. L’expérience du Pendjab à cet égard est importante pour comprendre des processus similaires dans les autres États et pour élaborer des stratégies appropriées pour les mouvements sociaux et le plaidoyer politique concernant les réformes agraires/​sociales.

Reflecting a longstanding intellectual heritage in Marxist political economy, contributions to agrarian studies have variously referred to the production, distribution and extraction of value. Despite this central role within the heritage of agrarian studies, the concept of value is often used inconsistently between authors and sometimes deployed without clear elucidation of the underlying theoretical tenets. As such, value often tends to be used more as a metaphor suggestive of conditions of exploitation rather than a detailed conceptual framework. In response, we must ask if there is still a robust case for value analysis forming a foundational pillar of agrarian studies? To address this challenging question, we invited three authors to give their perspective on the value of value for agrarian studies. First and foremost, we asked them to consider what value analysis does that is otherwise missed in critical agrarian studies and how we can mobilise its potential to sharpen analyses. Two further pivotal questions arise, spurred on by recent trends in the literature. First, to what extent do the categories of value enrich or hinder our evolving understanding of the dynamics of social reproduction within agrarian households and communities, including the gendered relations through which agriculture and livelihoods are performed? Similarly, are the largely anthropogenic concepts of value fit for the purpose of explaining environmental change and the more-than-human dynamics through which agricultural landscapes are produced and change over time?

Authors: A Haroon Akram-Lodhi, Srishti Yadav, Alessandra Mezzadri, Marcus Taylor

This article aims to understand the methodological position that Marx takes in Capital Volume 1 and the implications of that on the individual units in his analysis. With the understanding that Marx adopts a fundamentally holist methodological standpoint, the article outlines how the individuals within classes and the system of capitalist production are measured and contextualised. This question is answered by examining Marx’s Capital Volume 1 as the primary text. Where relevant, the researcher engages with Marx’s intellectual background and tradition. The individual units discussed in this study are the commodity, the worker and the capitalist.

This article examines how the concept of the representative individual emerges through averaging and how this process unfolds in Marx’s Capital, shaped by his methodological approach. The article illustrates the method of averaging in Marx, through his intellectual engagement with Quetelet as it also focuses on Hegel’s influence on Marx’s method and elaborates on the parallels and divergences between them. With the given engagement with Capital and Marx’s intellectual interactions, the researcher arrives at a specific understanding of holism that can be attributed to Marx in Capital Volume 1.

Author: Sushmita Rama Subrahmanyam, Student, MA in Economics (2024−2026)

This article aims to understand the methodological position that Marx takes in Capital Volume 1 and the implications of that on the individual units in his analysis. With the understanding that Marx adopts a fundamentally holist methodological standpoint, the article outlines how the individuals within classes and the system of capitalist production are measured and contextualised. This question is answered by examining Marx’s Capital Volume 1 as the primary text. Where relevant, the author engages with Marx’s intellectual background and tradition. The individual units discussed in this study are the commodity, the worker and the capitalist.

This article examines how the concept of the representative individual emerges through averaging and how this process unfolds in Marx’s Capital, shaped by his methodological approach. The article illustrates the method of averaging in Marx, through his intellectual engagement with Quetelet as it also focusses on Hegel’s influence on Marx’s method and elaborates on the parallels and divergences between them. With the given engagement with Capital and Marx’s intellectual interactions, we arrive at a specific understanding of holism that can be attributed to Marx in Capital Volume 1.

Author: Sushmita Rama Subrahmanyam

“Global learning crisis” narratives, in focusing on the ​“proximate determinants” of the crisis, represent a welcome ​“classroom turn” in international education and development. Extant learning crisis literatures are problematic, however, as their homogenizing gaze distorts how teachers and students co-constitute classrooms as locally meaningful learning spaces. Drawing on anthropological approaches in comparative education, this article addresses the ​“learning crisis” in a middle-school classroom in a weavers’ neighborhood in Kanchipuram. Constituted in an elaborate ​“notebook economy,” this classroom was an inventive response that not only accommodated students’ material cultures and social-educational disadvantages but also affected their belonging in a resource-scarce public education system. If the learning it afforded was disdained in ​“learning crisis” narratives, it was nevertheless relevant for students, readily translated into educationally unintensive assembly-line jobs. In producing contempt for such classrooms, ​“learning crisis” narratives merely distract from — and thus entrench — the deeply unequal economic and educational development that necessitated the notebook economy in the first place.

Articles featured in this issue highlight the importance of thoughtful, localised approaches to transforming India’s education system. Some examples being — Early Childhood Education: Towards a Practical Approach. The article argues for a play-based, holistic approach that supports young children as learners and individuals. A Decentralised Model of School Management explores how Rajasthan’s Panchayat Elementary Education Officer (PEEO) system strengthens local decision-making and drives school improvements. Revitalisation of Schools through Design — how smart, cost-effective design can turn classrooms into spaces that spark curiosity and support meaningful learning. AI in Public Education stresses the importance of integrating it thoughtfully, i.e., supporting educators and administrators in their work

Background

Mangrove species are vital to the ecosystems of tropical and subtropical coastlines worldwide. Despite the underexplored role of polyploidization in these species, deciphering its impact on gene expression is essential for understanding the connection between polyploidization and species diversification. Our initial investigation, integrating multiple nuclear loci with morphological and cytological data, indicates that the tetraploid Acanthus tetraploideus likely originated from allopolyploidization events involving the diploid species A. ilicifolius and A. ebracteatus. Expanding on these insights, this study utilises genome-wide evidence to confirm the divergence patterns among extant Acanthus mangrove diploids and to investigate the origin and transcriptome asymmetry of the tetraploid A. tetraploideus.

Results

Phylogenetic analysis and molecular dating revealed a closer evolutionary relationship between A. ebracteatus and A. volubilis than between A. ebracteatus and A. ilicifolius, diverged approximately 6.92 Mya and 9.59 Mya, respectively. Analysis of individual whole transcriptomes revealed that homeologous sequences in A. tetraploideus were preferentially clustered with A. ilicifolius and A. ebracteatus, rather than A. volubilis, in a roughly 1:1 ratio. The high similarity in nucleotide sequences and homologous polymorphisms between the tetraploid A. tetraploideus and its two parental diploids, A. ebracteatus and A. ilicifolius, supports the hypothesis of a recent allopolyploid origin for A. tetraploideus. Estimation of homeolog expression revealed a general attenuation of homeolog expression divergence in A. tetraploideus compared to the in silico parental mix, with 22.87% and 67.66% of genes exhibiting biased homeolog expression, respectively. Further investigation identified remarkable retention of parental expression dominance in the tetraploid, suggesting that parental genetic legacy substantially influences the reconfiguration of homeolog expression in the derived tetraploid. Meanwhile, the observation of numerous novel expression patterns between the two homeolog sets suggests that the transcriptome shock (i.e., the transcriptomic changes induced by interspecific hybridisation) associated with allopolyploidization and subsequent post-polyploid evolutionary processes also significantly impact transcriptome asymmetry in A. tetraploideus. While no strong evidence directly links transcriptomic changes to specific adaptive traits, the patterns in unbiased and novelly biased genes in A. tetraploideus suggest adaptations to stable polyploidy. Unbiased genes involved in fundamental cellular processes and novelly biased genes related to chromosome dynamics and cell cycle regulation may stabilise polyploid genomes, supporting the species’ establishment and long-term success. These findings underscore the role of transcriptomic stability in polyploid adaptation.

Conclusions

Our study sheds light on the evolutionary origins and the intricate transcriptional reconfiguration of the tetraploid A. tetraploideus. These insights significantly enhance our comprehension of the pivotal role that polyploidization plays in speciation and adaptative evolution of mangrove species.

Authors: Wuxia Guo, Achyut Kumar Banerjee, Hui Feng, Wei Lun Ng, Haidan Wu, Weixi Li, Yang Yuan & Yelin Huang